Branched polymers, pH and thermosensitive nanosystems for biomedical application

Previous and Current Research

We are focused on fundamental aspects of synthesis and characterization of branched uncharged polymers, polyelectrolytes and stimuli responsible branched polymers in solution as well as hydrogels and nanosystems synthesized in situ into these polymer matrices. We study the response of these complicated systems towards external stimuli ( pH, T ...) and their applications mainly in biomaterials or drug delivery fields.

Branched polymer matrices have various chemical nature and internal structure, higher local concentration of functional groups in comparison with their linear analogue, and they can be very efficient for designing of optimized nanocarriers-nanosystems for medical applications and for water treatment. We are working in collaboration with physicists and biologists.

Nanosystems for Photodynamic Therapy

Hydrogels for biomedical applications (PhD Nadtoka O)

It was shown that structure of hydrogels prepared by cross-linking of Dextran-graft-Polyacrylamide during synthesis had more possibility of Which?variation in comparison with cross-linked Polyacrylamide[12-13]. Polymeric hydrogels are used for biomedical applications as wound of various genesis dressing, artificial burn dressings, tissue technology, and water purification etc. [2]. The efficiency of such materials can be improved by incorporation of silver nanoparticles (AgNPs) which exhibit high antibacterial activity [3-4]. Size, size distribution (polydispersity), morphology, and surface charge of silver nanoparticles determine its biological activity [1] and are strongly dependent on the method of nanoparticles synthesis.

In the present work, we describe a new approach for synthesis and characterization of hydrogels based on cross-linked Dextran-graft-Polyacrylamide copolymers in non-ionic and anionic form with incorporated silver nanoparticles (AgNPs). 

Methodological and Technical Expertise

• Chemical modeling of branched polymers
• Synthesis of nanosystems into polymer matrices
• All experimental approaches for  characterization of polymers of complicated internal structure and nanosystems: elastic and quasy elastic light scattering, size-exclusion chromatography, transmission electron microscopy, differential scanning calorimetry, viscometry, potentiometric titration etc.

Chemists:

PhD V. Chumachenko

PhD student Yu. Harahuts

PhD O. Nadtoka

Biologist:

PhD P.Virych

 In collaboration with Physicists:

PhD A. Naumenko

Dr.Sci. O.Yeshchenko

Selected Publications

1.    Leonid Bulavin, Nataliya Kutsevol, Vasyl Chumachenko, Dmytro Soloviov, Alexander Kuklin, Andriy Marinin. SAXS combined with UV-vis spectroscopy and QUELS: accurate characterization of silver sols synthesized in polymer matrices. Nanoscale Research letters. 2016. 11:35.

2.    N. Kutsevol, V. Chumachenko, M. Rawiso, A. Shyichuk. Green synthesis of silver nanoparticles using glucose as reducing agent and dextran-graft-polyacrylamide as template. Micro & Nano letters, 2016, V.11, Issue 5, P. 256-259.

3.    V. A. Chumachenko, I. O. Shton, E. D. Shishko, N. V. Kutsevol, A. I. Marinin and N. F. Gamaleia. Branched Copolymers Dextran-Graft-Polyacrylamide as Nanocarriers for Delivery of Gold Nanoparticles and Photosensitizers to Tumor Cells. Сhapter in the Book: Nanophysics, Nanophotonics, Surface Studies, and Applications, 2016. Volume 183 of the series Springer Proceedings in Physics/ pp 379-390.  Eds:Olena Fesenko, Leonid Yatsenko

4.    V. Chumachenko, N. Kutsevol, Yu. Harahuts, M. Rawiso, A. Marinin, L. Bulavin. Star-like Dextran-graft-PNiPAM copolymers. Effect of internal molecular structure on the phase transition. Journal of Molecular Liquids. 2017. V.235, P.77-82.

5.    G. Telegeev, N. Kutsevol, V. Chumachenko, A. Naumenko, P. Telegeeva, S. Filipchenko, and Yu. Harahuts. Dextran-Polyacrylamide as Matrices for Creation of Anticancer Nanocomposite. International Journal of Polymer Science. 2017.  Article ID 4929857.

6.    Oleg A. Yeshchenko, Antonina P. Naumenko, Nataliya V. Kutsevol, Daria O. Maskova, Iulia I. Harahuts, Vasyl A. Chumachenko, Andrey I. Marinin. Anomalous Inverse Hysteresis of Phase Transition in Thermosensitive Dextran-graft-PNIPAM Copolymer/Au Nanoparticles Hybrid Nanosystem. J. Phys. Chem. C, 2018, 122 (14), pp 8003–8010.

7.    N. Kutsevol, A. Glamazda, Chumachenko,Yu. Harahuts, S. G. Stepanian, A. M. Plokhotnichenko, V. A. Karachevtsev. Behavior of hybrid thermosensitive nanosystem dextran-graft-pnipam/gold nanoparticles: characterization within LCTS. Journal of Nanoparticle Research.2018.  J Nanopart Res (2018) 20: 236.

8.    Tatiana Matvienko, Viktoriya Sokolova, Svitlana Prylutska, Yuliia Harahuts, Nataliya Kutsevol, Viktor Kostjukov, Maxim Evstigneev, Yuriy Prylutskyy, Matthias Epple, Uwe Ritter. In vitro study of the anticancer activity of various Doxorubicin-containing dispersions. Bioimpacts. 2019;9(1):59-70.

9.    O. Nadtoka, N. Kutsevol,·A. Naumenko, P. Virych. Photochemical synthesis and characterization of hydrogel–silver nanoparticle composites. Research on Chemical Intermediates. 2019.

10. N. Kutsevol, A. Naumenko, Yu. Harahuts, V. Chumachenko, I. Shton, E. Shishko, N. Lukianova, V. Chekhun. New hybrid composites for photodynamic therapy: synthesis, characterization and biological study.  Appl Nanoscience. 2019. 9: 881. 

(Scopus) Kutsevol, Nataliya V. Author ID: 6603211250

Contacts